Journal: Journal of Cancer Research and Clinical Oncology
Article Title: Immunogenic dying cells elicit potent anti-tumor T cell immunity against lung metastasis and tumorigenesis
doi: 10.1007/s00432-025-06087-z
Figure Lengend Snippet: Immunization of the ICD dying tumor cells activates Th1 cells in tumor microenvironment. A . Schema of the immunization experiment to isolate lymphocytes from tumor tissue for analysis ( n = 5). B . IFNγ and IL-4 in in the axillary LNs (L) of different groups ( n = 5) by ELISA. C . Schema of the immunization experiment to isolate lymphocytes from tumor tissue for analysis ( n = 5). D . IFN-γ and IL-4 in tumor tissues (M) of different groups by ELISA. E-H . Flow cytometry analysis for the content and distribution of immune cells in tumor tissues as indicated. ** p < 0.01, *** p < 0.001, **** p < 0.0001
Article Snippet: All antibody information is shown following: PE Rat Anti-CD11b (553311, BD), Alexa Fluor ® 647 Rat Anti-Mouse F4/80 (565854, BD) BD HorizonTM PE-CF594 Rat Anti-Mouse IFN-γ antibody (5562333 BD).
Techniques: Enzyme-linked Immunosorbent Assay, Flow Cytometry